Plasmid

Part:BBa_K4687008:Design

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-10-02)


pXMJ19


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5749
    Illegal XbaI site found at 5722
    Illegal SpeI site found at 1965
    Illegal SpeI site found at 2344
    Illegal PstI site found at 5710
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5749
    Illegal SpeI site found at 1965
    Illegal SpeI site found at 2344
    Illegal PstI site found at 5710
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5749
    Illegal BamHI site found at 5728
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5749
    Illegal XbaI site found at 5722
    Illegal SpeI site found at 1965
    Illegal SpeI site found at 2344
    Illegal PstI site found at 5710
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5749
    Illegal XbaI site found at 5722
    Illegal SpeI site found at 1965
    Illegal SpeI site found at 2344
    Illegal PstI site found at 5710
    Illegal NgoMIV site found at 3638
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Plasmid vectors introduced the CRISPR components into the cells to ensure that they function properly within the cell. By selecting different plasmid vectors for experiments, the most suitable ones were selected to improve the efficiency of gene editing.


Source

E. coli-C. glutamicum shuttle expression vector.

References